Cusabio
SKU(재고 관리 코드):CSB-MA017667A0m
PD-L2 Monoclonal Antibody
PD-L2 Monoclonal Antibody
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| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-MA017667A0m |
| Short Name | PD-L2 Monoclonal Antibody |
| Antibody Type | Monoclonal Antibody |
| List Price | $350/100ul, $210/50ul |
| Species Reactivity | Human |
| Tested Applications | ELISA, WB, IHC, IF, FC, IP |
| Dilution | WB:1:5000-1:640000, IHC:1:50-1:200, IF:1:50-1:200, FC:1:50-1:200, IP:2µl-5µl |
| Purification Method | Protein A purification |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | Recombinant human Programmed cell death 1 ligand 2 protein (21-118aa) |
| Antigen Species | Human |
| Uniprot ID | Q9BQ51 |
| Target Name | PD-L2 |
| Clonality | Monoclonal |
| Clone ID | 7F11D11 |
| Host Species | Mouse |
| Isotype | IgG2b |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12928636/ |
| Size | 100ul |
Image 1
Western Blot
Positive WB detected in: HepG2 whole cell lysate, Hela whole cell lysate, PC-3 whole cell lysate, A549 whole cell lysate
All lanes PD-L2 antibody at 1:2000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 31,21 KDa
Observed band size: 45-50 KDa
Exposure time：5min
Image 2
Western Blot
Positive WB detected in: Hela whole cell lysate at 20μg, 10μg, 5μg, 2.5μg, 1.25μg
All lanes: PD-L2 antibody at 1:2000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 31,21 KDa
Observed band size: 45-50 KDa
Exposure time：5min
Image 3
Western Blot
Positive WB detected in: 15μg hela whole cell lysate
PD-L2 antibody at 1:5000, 1:1W, 1:2W, 1:4W, 1:8W, 1:16W, 1:32W, 1:64W
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 31,21 KDa
Observed band size: 45-50 KDa
Exposure time：5min
Image 4
IHC image of CSB-MA017667A0m diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Image 5
Immunofluorescence staining of HepG2 cells with CSB-MA017667A0m at 1:100, counter-stained with DAPI. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
Image 6
Immunofluorescence staining of Hela cells with CSB-MA017667A0m at 1:100, counter-stained with DAPI. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
Image 7
Immunofluorescence staining of Raji cells with CSB-MA017667A0m at 1:100, counter-stained with DAPI. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
Image 8
Immunofluorescence staining of U251 cells with CSB-MA017667A0m at 1:100, counter-stained with DAPI. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
Image 9
Overlay histogram showing 293 cells transfected with PD-L2 stained with CSB-MA017667A0m (red line). The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (2µg/1*10<sup>6</sup> cells) for 1 h at 4°C. The secondary antibody used was R-PE-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG2b (2µg/1*10<sup>6</sup> cells) used under the same conditions. Acquisition of >10,000 events was performed.
Image 10
Immunoprecipitating PD-L2 in Hela whole cell lysate
Lane 1: Mouse control IgG instead of CSB-MA017667A0m in Hela whole cell lysate.
Lane 2: CSB-MA017667A0m (2µl) + Hela whole cell lysate (500µg)
Lane 3: Hela whole cell lysate (20µg)
For western blotting, the blot was detected with CSB-MA017667A0m at 1:2000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:2000
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