Cusabio
SKU(재고 관리 코드):CSB-MA018072A0m
PKM Monoclonal Antibody
PKM Monoclonal Antibody
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| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-MA018072A0m |
| Short Name | PKM Monoclonal Antibody |
| Antibody Type | Monoclonal Antibody |
| List Price | $350/100ul, $210/50ul |
| Species Reactivity | Human, Rat, Mouse, Rabbit |
| Tested Applications | ELISA, WB, IHC, IF, FC, IP |
| Dilution | WB:1:4000-1:256000, IHC:1:200-1:500, IF:1:150-1:300, FC:1:50-1:200, IP:1µl-4µl |
| Purification Method | Protein G purification |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | Recombinant human Pyruvate kinase PKM protein (2-531aa) |
| Antigen Species | Human |
| Uniprot ID | P14618 |
| Target Name | PKM |
| Clonality | Monoclonal |
| Clone ID | 6C3C7 |
| Host Species | Mouse |
| Isotype | IgG1 |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12928558/ |
| Size | 100ul |
Image 1
Western Blot
Positive WB detected in: A549 whole cell lysate, 293T whole cell lysate, U251 whole cell lysate, Rat Brain tissue, Rabbit Skeletal Muscle tissue
All lanes: PKM antibody at 1:1000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 58 kDa
Observed band size: 58 KDa
Exposure time: 1min
Image 2
Western Blot
Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate, NIH/3T3 whole cell lysate
All lanes: PKM antibody at 1:1000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 58 kDa
Observed band size: 58 KDa
Exposure time: 1min
Image 3
Western Blot
Positive WB detected in: Mouse Heart tissue, Mouse Brain tissue, Mouse Skeletal Muscle tissue
All lanes: PKM antibody at 1:4000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 55-60 kDa
Observed band size: 55-60 kDa
Image 4
Western Blot
Positive WB detected in: Rat Heart tissue, Rat Spleen tissue, Rat Brain tissue
All lanes: PKM antibody at 1:4000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 55-60 kDa
Observed band size: 55-60 kDa
Image 5
Western Blot
Positive WB detected in: MCF-7 whole cell lysate at 40µg, 20µg, 10µg, 5µg, 2.5µg, 1.25µg, 0.625µg, 0.3125µg
All lanes: PKM antibody at 1:1000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 58 kDa
Observed band size: 58 KDa
Exposure time: 5min
Image 6
Western Blot
Positive WB detected in: MCF-7 whole cell lysate
All lanes: PKM antibody at 1:4000, 1:8000, 1:16000, 1:32000, 1:64000, 1:128000, 1:256000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 58 kDa
Observed band size: 58 KDa
Exposure time: 5min
Image 7
IHC image of CSB-MA018072A0m diluted at 1:400 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Image 8
IHC image of CSB-MA018072A0m diluted at 1:400 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Image 9
IHC image of CSB-MA018072A0m diluted at 1:400 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Image 10
IHC image of CSB-MA018072A0m diluted at 1:400 and staining in paraffin-embedded human lung cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Image 11
IHC image of CSB-MA018072A0m diluted at 1:400 and staining in paraffin-embedded human lung cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Image 12
IHC image of CSB-MA018072A0m diluted at 1:400 and staining in paraffin-embedded human lung cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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