Cusabio
SKU(재고 관리 코드):CSB-MA019049A0m
CD45 Monoclonal Antibody
CD45 Monoclonal Antibody
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| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-MA019049A0m |
| Short Name | CD45 Monoclonal Antibody |
| Antibody Type | Monoclonal Antibody |
| List Price | $350/100ul, $210/50ul |
| Species Reactivity | Human |
| Tested Applications | ELISA, WB, IHC, IF, FC |
| Dilution | WB:1:2000-1:32000, IHC:1:200-1:600, IF:1:100-1:300, FC:1:200-1:600 |
| Purification Method | Protein A purification |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | Recombinant human Receptor-type tyrosine-protein phosphatase C protein (24-575aa) |
| Antigen Species | Human |
| Uniprot ID | P08575 |
| Target Name | CD45 |
| Clonality | Monoclonal |
| Clone ID | 17H9D4 |
| Host Species | Mouse |
| Isotype | IgG2b |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12928654/ |
| Size | 100ul |
Image 1
Western Blot
Image 2
Western Blot
Image 3
Western Blot
Image 4
Western Blot
Image 5
IHC image of CSB-MA019049A0m diluted at 1:500 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG labeled by HRP and visualized using 0.05% DAB.
Image 6
IHC image of CSB-MA019049A0m diluted at 1:500 and staining in paraffin-embedded human lymph node tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG labeled by HRP and visualized using 0.05% DAB.
Image 7
Immunofluorescence staining of Jurkat cells with CSB-MA019049A0m at 1:250, counter-stained with DAPI. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
Image 8
Immunofluorescence staining of Raji cells with CSB-MA019049A0m at 1:250, counter-stained with DAPI. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
Image 9
Immunofluorescence staining of U937 cells with CSB-MA019049A0m at 1:250, counter-stained with DAPI. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
Image 10
Overlay histogram showing Jurkat cells stained with CSB-MA019049A0m (red line) at 1:500. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*10<sup>6</sup> cells) for 1 h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG2b (1µg/1*10<sup>6</sup> cells) used under the same conditions. Acquisition of >10,000 events was performed.
Image 11
Overlay histogram showing Raji cells stained with CSB-MA019049A0m (red line) at 1:500. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*10<sup>6</sup> cells) for 1 h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG2b (1µg/1*10<sup>6</sup> cells) used under the same conditions. Acquisition of >10,000 events was performed.
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