Cusabio
SKU(재고 관리 코드):CSB-MA021780A0m
SLN Monoclonal Antibody
SLN Monoclonal Antibody
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| Release Date | 2023-01-18 |
|---|---|
| Product Code | CSB-MA021780A0m |
| Short Name | SLN Monoclonal Antibody |
| Antibody Type | Monoclonal Antibody |
| List Price | $350/100ul, $210/50ul |
| Species Reactivity | Human |
| Tested Applications | ELISA, IHC, FC |
| Dilution | IHC:1:50-1:200, FC:1:50-1:200 |
| Purification Method | Protein G purification |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | Recombinant human SLN protein (1-31aa) |
| Antigen Species | Human |
| Uniprot ID | O00631 |
| Target Name | SLN |
| Clonality | Monoclonal |
| Clone ID | 14G1A12 |
| Host Species | Mouse |
| Isotype | IgG2b |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12932135/ |
| Size | 50ul |
Image 1
IHC image of CSB-MA021780A0m diluted at 1:60 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Image 2
Overlay Peak curve showing U87 cells stained with CSB-MA021780A0m (red line) at 1:60. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
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