Cusabio
SKU(재고 관리 코드):CSB-MA023974A1m
TNFRSF17 Monoclonal Antibody
TNFRSF17 Monoclonal Antibody
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| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-MA023974A1m |
| Short Name | TNFRSF17 Monoclonal Antibody |
| Antibody Type | Monoclonal Antibody |
| List Price | $350/100ul, $210/50ul |
| Species Reactivity | Human |
| Tested Applications | ELISA, WB, IF, FC |
| Dilution | WB:1:1000-1:5000, IF:1:50-1:200, FC:1:50-1:200 |
| Purification Method | Protein G purification |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | Recombinant human TNFRSF17 protein (1-54aa) |
| Antigen Species | Human |
| Uniprot ID | Q02223 |
| Target Name | TNFRSF17 |
| Clonality | Monoclonal |
| Clone ID | 10A9F7 |
| Host Species | Mouse |
| Isotype | IgG2b |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12929121/ |
| Size | 100ul |
Image 1
Western Blot
Positive WB detected in: 1-2 lanes: 293F whole cell lysate transfected with BCMA; 3-4 lane: Recombinant proteins with BCMA
All lanes: TNFRSF17 antibody at 1:1000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 40, 15 KDa
Observed band size: 40, 15 KDa
Exposure time：10min
Image 2
Immunofluorescence staining of 293F cells transfected with TNFRSF17 with CSB-MA023974A1m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was R-PE-conjugated Goat Anti-Mouse IgG(H+L).
Image 3
Overlay histogram showing 293F cells transfected with TNFRSF17 stained with CSB-MA023974A1m (red line) at 1:100. The cells were fixed in 70% ethanol at 4℃ overnight. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*10<sup>6</sup> cells) for 1 h at 4°C. The secondary antibody used was Alexa Fluor 647 AffiniPure Donkey Anti-Mouse IgG (H+L) at 1/250 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG2b (1µg/1*10<sup>6</sup> cells) used under the same conditions. Acquisition of >10,000 events was performed.
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