Cusabio
SKU(재고 관리 코드):CSB-MA025318A0m
TUBB Monoclonal Antibody
TUBB Monoclonal Antibody
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| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-MA025318A0m |
| Short Name | TUBB Monoclonal Antibody |
| Antibody Type | Monoclonal Antibody |
| List Price | $200/100ul, $120/50ul |
| Species Reactivity | Human, Rat, Rabbit, Mouse |
| Tested Applications | ELISA, WB, IHC, IF, FC, IP |
| Dilution | WB:1:5000-1:640000, IHC:1:100-1:300, IF:1:50-1:200, FC:1:100-1:300, IP:1µl-2µl |
| Purification Method | Protein A purification |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | GAGNNWAKGHYTEGA synthetic peptide conjugate to KLH |
| Antigen Species | Species Independent |
| Target Name | TUBB |
| Clonality | Monoclonal |
| Clone ID | 16E11D4 |
| Host Species | Mouse |
| Isotype | IgG2b |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12929074/ |
| Size | 50ul |
Image 1
Western Blot
Positive WB detected in: 293 whole cell lysate, A549 whole cell lysate, Hela whole cell lysate, MCF-7 whole cell lysate
All lanes TUBB antibody at 1:5000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 55 KDa
Observed band size: 55 KDa
Exposure time:5s
Image 2
Western Blot
Positive WB detected in: 293 whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate , Rat kidney tissue , Rat stomach tissue
All lanes TUBB antibody at 1:5000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 55 KDa
Observed band size: 55 KDa
Exposure time:5min
Image 3
Western Blot
Positive WB detected in: Rat heart tissue ,Rat liver tissue, Mouse heart tissue, Mouse liver tissue, Rat brain tissue, Mouse brain tissue, Raw264.7 whole cell lysate, A375 whole cell lysate
All lanes TUBB antibody at 1:5000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 55 KDa
Observed band size: 55 KDa
Exposure time:5min
Image 4
Western Blot
Positive WB detected in: Mouse heart tissue, Rabbit heart tissue, Rabbit liver tissue, Rabbit lung tissue, Rabbit kidney tissue, Rabbit spleen tissue
All lanes TUBB antibody at 1:5000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 55 KDa
Observed band size: 55 KDa
Exposure time:5min
Image 5
Western Blot
Positive WB detected in: 20μg hela whole cell lysate TUBB antibody at 1:5000, 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 1:320000, 1:640000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 55 KDa
Observed band size: 55 KDa
Exposure time:5min
Image 6
Western Blot
Positive WB detected in: Hela whole cell lysate at 20μg, 10μg, 5μg, 2.5μg, 1.25μg, 0.625μg, 0.3125μg All lanes:TUBB antibody at 1:5000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 55 KDa
Observed band size: 55 KDa
Exposure time:5min
Image 7
IHC image of CSB-MA025318A0m diluted at 1:200 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37掳C Then primary antibody (1% BSA) was incubated at 4掳C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
Image 8
IHC image of CSB-MA025318A0m diluted at 1:200 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37掳C Then primary antibody (1% BSA) was incubated at 4掳C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
Image 9
IHC image of CSB-MA025318A0m diluted at 1:200 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37掳C Then primary antibody (1% BSA) was incubated at 4掳C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
Image 10
IHC image of CSB-MA025318A0m diluted at 1:200 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37掳C Then primary antibody (1% BSA) was incubated at 4掳C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
Image 11
IHC image of CSB-MA025318A0m diluted at 1:200 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37掳C Then primary antibody (1% BSA) was incubated at 4掳C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
Image 12
Immunofluorescence staining of NIH/3T3 cells with CSB-MA025318A0m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4掳C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
Image 13
Immunofluorescence staining of A549 cells with CSB-MA025318A0m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4掳C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
Image 14
Immunofluorescence staining of Hela cells with CSB-MA025318A0m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4掳C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
Image 15
Immunofluorescence staining of HepG2 cells with CSB-MA025318A0m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4掳C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
Image 16
Overlay Peak curve showing A549 cells stained with CSB-MA025318A0m (red line) at 1:200. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1碌g/1*10<sup>6</sup>cells) for 1 h at 4掳C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4掳C. Isotype control antibody (green line) was mouse IgG2b (1碌g/1*10<sup>6</sup> cells) used under the same conditions. Acquisition of >10,000 events was performed.
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