Cusabio
SKU(재고 관리 코드):CSB-MA234468A0m
Whitlow linker Monoclonal Antibody
Whitlow linker Monoclonal Antibody
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| Release Date | 2024-10-14 |
|---|---|
| Product Code | CSB-MA234468A0m |
| Short Name | Whitlow linker Monoclonal Antibody |
| Antibody Type | Monoclonal Antibody |
| List Price | $600/100ul, $329/50ul |
| Species Reactivity | Human |
| Tested Applications | ELISA, WB, FC |
| Dilution | WB:1:1000-1:5000, FC:1:50-1:200 |
| Purification Method | Protein G purification |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | Whitlow linker-KLH Conjugate |
| Antigen Species | Species Independent |
| Target Name | Whitlow linker |
| Clonality | Monoclonal |
| Clone ID | 18A3G5 |
| Host Species | Mouse |
| Isotype | IgG2b |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12937984/ |
| Size | 100ul |
Image 1
The Binding Activity of Whitlow linker with Anti-Whitlow linker antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized scFv-based Anti-CD19 CAR recombinant antibody at 2 μg/mL can bind Anti-Whitlow linker antibody. The EC<sub>50</sub> is 5.147 to 5.761 ng/mL.
Image 2
Western Blot
Positive WB detected in: 293F whole cell lysate, scFv-based Anti-CD19 CAR containing a Whitlow linker (293F/CD19 CAR) whole cell lysate
All lanes: Whitlow linker antibody at 1:1000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 50-200 kDa
Observed band size: 50-200 KDa
Exposure time：5s
Image 3
Untransfected HEK-293F cells (green line) and transfected scFv-based Anti-CD19 CAR containing a Whitlow/218 linker stable cells (red line) were stained with anti-Whitlow linker antibody (2µg/1*10<sup>6</sup>cells), washed and then followed by FITC-conjugated anti-Mouse IgG Fc antibody and analyzed with flow cytometry.
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