Cusabio
SKU(재고 관리 코드):CSB-MA621519A0m
MERTK Monoclonal Antibody
MERTK Monoclonal Antibody
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| Release Date | 2023-01-18 |
|---|---|
| Product Code | CSB-MA621519A0m |
| Short Name | MERTK Monoclonal Antibody |
| Antibody Type | Monoclonal Antibody |
| List Price | $350/100ul, $210/50ul |
| Species Reactivity | Human |
| Tested Applications | ELISA, WB, IF, FC |
| Dilution | WB:1:1000-1:5000, IF:1:50-1:200, FC:1:50-1:200 |
| Purification Method | Protein A purification |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | Recombinant MERTK protein (21-505aa) |
| Antigen Species | Human |
| Uniprot ID | Q12866 |
| Target Name | MERTK |
| Clonality | Monoclonal |
| Clone ID | 16D1 |
| Host Species | Mouse |
| Isotype | IgG2b |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12931933/ |
| Size | 100ul |
Image 1
Western Blot
Positive WB detected in: 1-4 lanes: 293T whole cell lysate transfected with MERTK at 10ug, 5ug, 2.5ug, 1.25ug
All lanes: MERTK antibody at 1:2000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 150-250 KDa
Observed band size: 150-250 KDa
Exposure time：5S
Image 2
Western Blot
Positive WB detected in: 1-4 lanes: Recombinant MERTK protein at 20ng, 10ng, 5ng, 2.5ng
All lanes: MERTK antibody at 1:2000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 120 KDa
Observed band size: 120-150 KDa
Exposure time：5S
Image 3
Western Blot
Positive WB detected in: 293 whole cell lysate, SY5Y whole cell lysate, K562 whole cell lysate
All lanes: MERTK antibody at 1:1000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 110 kDa
Observed band size: 130 KDa
Exposure time：5min
Image 4
Immunofluorescence staining of 293T transfected cells with MERTK CSB-MA621519A0m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
Image 5
Immunofluorescence staining of HepG2 cells with MERTK CSB-MA621519A0m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).
Image 6
Overlay Peak curve showing 293T transfected cells stained with CSB-MA621519A0m (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*10<sup>6</sup> cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*10<sup>6</sup> cells) used under the same conditions. Acquisition of >10,000 events was performed.
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