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Cusabio

SKU(재고 관리 코드):CSB-MA731035A0m

MERTNL Monoclonal Antibody

MERTNL Monoclonal Antibody

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통합 견적 요청
Size
Release Date 2023-01-18
Product Code CSB-MA731035A0m
Short Name MERTNL Monoclonal Antibody
Antibody Type Monoclonal Antibody
List Price $350/100ul, $210/50ul
Species Reactivity Human
Tested Applications ELISA, IHC, FC
Dilution IHC:1:50-1:200, FC:1:50-1:200
Purification Method Protein A purification
Buffer Formulation 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4.
Antigen Recombinant human Meteorin-like protein (46-311aa)
Antigen Species Human
Uniprot ID Q641Q3
Target Name MERTNL
Clonality Monoclonal
Clone ID 1F7A6
Host Species Mouse
Isotype IgG2a
Conjugation Non-conjugated
Form Liquid
Storage Condition Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
TDS URL https://www.cusabio.com/datasheet/12932136/
Size 50ul

Image 1

IHC image of CSB-MA731035A0m diluted at 1:50 and staining in paraffin-embedded human skin tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Image 2

Overlay Peak curve showing Hela cells stained with CSB-MA731035A0m (red line) at 1:50. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.

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