Cusabio
SKU(재고 관리 코드):CSB-MA835707A0m
SEMA4D Monoclonal Antibody
SEMA4D Monoclonal Antibody
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| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-MA835707A0m |
| Short Name | SEMA4D Monoclonal Antibody |
| Antibody Type | Monoclonal Antibody |
| List Price | $350/100ul, $210/50ul |
| Species Reactivity | Human |
| Tested Applications | ELISA, WB, FC |
| Dilution | WB:1:1000-1:5000, FC:1:50-1:200 |
| Purification Method | Protein G purification |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | Recombinant human SEMA4D protein(22-734aa) |
| Antigen Species | Human |
| Uniprot ID | Q92854 |
| Target Name | SEMA4D |
| Clonality | Monoclonal |
| Clone ID | 10E12E10 |
| Host Species | Mouse |
| Isotype | IgG2b |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12931887/ |
| Size | 100ul |
Image 1
Western Blot
Positive WB detected in: 293T whole cell lysate transfected with SEMA4D; Recombinant proteins with SEMA4D
All lanes: SEMA4D antibody at 1:1000
Secondary
Goat polyclonal to mouse IgG at 1/50000 dilution
Predicted band size: 130, 150 KDa
Observed band size: 130, 150 KDa
Exposure time：5min
Image 2
Overlay Peak curve showing 293T whole cell lysate transfected with SEMA4D stained with CSB-MA835707A0m (red line) at 1:100. The cells were incubated in 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*10<sup>6</sup> cells) for 1h at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*10<sup>6</sup> cells) used under the same conditions. Acquisition of >10,000 events was performed.
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