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Cusabio

SKU(재고 관리 코드):CSB-PA001900HA01HU

APEX1 Antibody

APEX1 Antibody

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통합 견적 요청
Size
Release Date 2022-04-01
Product Code CSB-PA001900HA01HU
Short Name APEX1 Antibody
Antibody Type Polyclonal Antibody
List Price $299/100ug, $166/50ug
Species Reactivity Human, Mouse
Tested Applications ELISA, WB, IHC, ChIP
Dilution WB:1:1000-1:5000, IHC:1:20-1:200
Purification Method Protein G purification
Buffer Formulation 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4.
Antigen Recombinant human DNA-(apurinic or apyrimidinic site) lyase protein (32-318aa)
Antigen Species Human
Uniprot ID P27695
Target Name APEX1
Clonality Polyclonal
Host Species Rabbit
Isotype IgG
Conjugation Non-conjugated
Form Liquid
Storage Condition Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
TDS URL https://www.cusabio.com/datasheet/172415/
Size 50ug

Image 1

Western blot

All lanes: APEX1 antibody at 2µg/ml

Lane 1: Hela whole cell lysate

Lane 2: Mouse brain tissue

Lane 3: MCF-7 whole cell lysate

Lane 4: A431 whole cell lysate

Secondary

Goat polyclonal to rabbit IgG at 1/15000 dilution

Predicted band size: 36 kDa

Observed band size: 36 kDa

Image 2

Immunohistochemistry of paraffin-embedded human cervical cancer using CSB-PA001900HA01HU at dilution of 1:100

Image 3

Chromatin Immunoprecipitation MCF-7 (1.1*10<sup>6</sup>) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4µg anti-APEX1 or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers (CSB-PP001900HU) against the MDR1 promoter.

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