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Cusabio

SKU(재고 관리 코드):CSB-PA004364HA01HU

CA1 Antibody

CA1 Antibody

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통합 견적 요청
Size
Release Date 2022-04-01
Product Code CSB-PA004364HA01HU
Short Name CA1 Antibody
Antibody Type Polyclonal Antibody
List Price $299/100ug, $166/50ug
Species Reactivity Human, Mouse
Tested Applications ELISA, WB, IHC, IF, IP
Dilution WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200, IP:1:200-1:2000
Purification Method Protein G purification
Buffer Formulation 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4.
Antigen Recombinant human Carbonic anhydrase 1 protein (2-261aa)
Antigen Species Human
Uniprot ID P00915
Target Name CA1
Clonality Polyclonal
Host Species Rabbit
Isotype IgG
Conjugation Non-conjugated
Form Liquid
Storage Condition Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
TDS URL https://www.cusabio.com/datasheet/1028809/
Size 50ug

Image 1

Immunohistochemistry of paraffin-embedded human melanoma using CSB-PA004364HA01HU at dilution of 1:100

Image 2

Immunofluorescent analysis of A549 cells using CSB-PA004364HA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Image 3

Western Blot

Positive WB detected in: K562 whole cell lysate, Mouse spleen tissue, Mouse kidney tissue

All lanes: CA1 antibody at 6.5µg/ml

Secondary

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 29 kDa

Observed band size: 29 kDa

Image 4

Immunoprecipitating CA1 in K562 whole cell lysate

Lane 1: Rabbit control IgG instead of CSB-PA004364HA01HU in K562 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)

Lane 2: CSB-PA004364HA01HU (8µg) + K562 whole cell lysate (500µg)

Lane 3: K562 whole cell lysate (20µg)

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