Cusabio
SKU(재고 관리 코드):CSB-PA005941DSR1HU
CRB1 Antibody
CRB1 Antibody
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| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-PA005941DSR1HU |
| Short Name | CRB1 Antibody |
| Antibody Type | Polyclonal Antibody |
| List Price | $299/100ul, $166/50ul |
| Species Reactivity | Human |
| Tested Applications | ELISA, IHC, IF |
| Dilution | IHC:1:20-1:200, IF:1:10-1:100 |
| Purification Method | Antigen affinity purification |
| Buffer Formulation | PBS, 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Antigen | Recombinant human Protein crumbs homolog 1 protein (20-200aa) |
| Antigen Species | Human |
| Uniprot ID | P82279 |
| Target Name | CRB1 |
| Clonality | Polyclonal |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/11909915/ |
| Size | 50ul |
Image 1
IHC image of CSB-PA005941DSR1HU diluted at 1:66 and staining in paraffin-embedded human eye tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Image 2
Immunofluorescence staining of Hela cells with CSB-PA005941DSR1HU at 1:22, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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