Cusabio
SKU(재고 관리 코드):CSB-PA010100OA04acHU
Acetyl-H2AFZ (K4) Antibody
Acetyl-H2AFZ (K4) Antibody
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| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-PA010100OA04acHU |
| Short Name | Acetyl-H2AFZ (K4) Antibody |
| Antibody Type | Polyclonal Antibody |
| List Price | $319/100ul, $175/50ul |
| Species Reactivity | Human |
| Tested Applications | ELISA, WB, ICC, IF, IP, ChIP |
| Dilution | WB:1:200-1:2000, ICC:1:10-1:100, IF:1:10-1:100, IP:1:200-1:2000 |
| Purification Method | Antigen affinity purification |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | Peptide sequence around site of Acetyl-Lys (4) derived from human Histone H2A.Z |
| Antigen Species | Human |
| Uniprot ID | P0C0S5 |
| Target Name | H2AFZ |
| Clonality | Polyclonal |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12782937/ |
| Size | 100ul |
Image 1
Western Blot
Positive WB detected in: K562 whole cell lysate (treated by 30mM sodium butyrate for 4h)
All lanes: H2AFZ antibody at 1.06µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 14 kDa
Observed band size: 14 kDa
Image 2
Immunofluorescence staining of Hela cells with CSB-PA010100OA04acHU at 1:10, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Image 3
Chromatin Immunoprecipitation Hela (4*10<sup>6</sup>, treated with 30mM sodium butyrate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-H2AFZ (CSB-PA010100OA04acHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
Image 4
Immunocytochemistry analysis of CSB-PA010100OA04acHU diluted at 1:20 and staining in Hela cells (treated with 30mM sodium butyrate for 4h) performed on a Leica BondTM system. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Image 5
Immunoprecipitating H2AFZ in HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h)
Lane 1: Rabbit control IgG instead of CSB-PA010100OA04acHU in HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h). For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA010100OA04acHU (5µg) + HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h) (500µg)
Lane 3: HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h) (20µg)
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