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Cusabio

SKU(재고 관리 코드):CSB-PA010100OA07nacHU

H2AFZ (Ab-7) Antibody

H2AFZ (Ab-7) Antibody

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통합 견적 요청
Size
Release Date 2022-04-01
Product Code CSB-PA010100OA07nacHU
Short Name H2AFZ (Ab-7) Antibody
Antibody Type Polyclonal Antibody
List Price $319/100ul, $175/50ul
Species Reactivity Human
Tested Applications ELISA, WB, IHC
Dilution WB:1:200-1:2000, IHC:1:20-1:200
Purification Method Antigen affinity purification
Buffer Formulation 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4.
Antigen Peptide sequence around site of Lys (7) derived from human Histone H2A.Z
Antigen Species Human
Uniprot ID P0C0S5
Target Name H2AFZ
Clonality Polyclonal
Host Species Rabbit
Isotype IgG
Conjugation Non-conjugated
Form Liquid
Storage Condition Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
TDS URL https://www.cusabio.com/datasheet/12782942/
Size 50ul

Image 1

Western Blot

Positive WB detected in: HepG2 whole cell lysate, A549 whole cell lysate, K562 whole cell lysate, HL60 whole cell lysate

All lanes: H2AFZ antibody at 0.8µg/ml

Secondary

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 14 kDa

Observed band size: 14 kDa

Image 2

IHC image of CSB-PA010100OA07nacHU diluted at 1:50 and staining in paraffin-embedded human prostate tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Image 3

IHC image of CSB-PA010100OA07nacHU diluted at 1:50 and staining in paraffin-embedded human small intestine tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

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