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Cusabio

SKU(재고 관리 코드):CSB-PA010100PA11acHU

Acetyl-H2AFZ (K11) Antibody

Acetyl-H2AFZ (K11) Antibody

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통합 견적 요청
Size
Release Date 2022-04-01
Product Code CSB-PA010100PA11acHU
Short Name Acetyl-H2AFZ (K11) Antibody
Antibody Type Polyclonal Antibody
List Price $319/100ul, $175/50ul
Species Reactivity Human
Tested Applications ELISA, WB, IF, ChIP
Dilution WB:1:200-1:2000, IF:1:50-1:200
Purification Method Antigen affinity purification
Buffer Formulation 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4.
Antigen Peptide sequence around site of Acetyl-Lys (11) derived from human Histone H2A.Z
Antigen Species Human
Uniprot ID P0C0S5
Target Name H2AFZ
Clonality Polyclonal
Host Species Rabbit
Isotype IgG
Conjugation Non-conjugated
Form Liquid
Storage Condition Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
TDS URL https://www.cusabio.com/datasheet/12782903/
Size 100ul

Image 1

Immunofluorescent analysis of Hela cells (sodium butyrate, 30 mM, 4h) using CSB-PA010100PA11acHU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Image 2

Chromatin Immunoprecipitation Hela (4*10<sup>6</sup>, treated with 30mM sodium butyrate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 8µg anti-H2AFZ (CSB-PA010100PA11acHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.

Image 3

Western Blot

Positive WB detected in: HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h)

All lanes: H2AFZ antibody at 1µg/ml

Secondary

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 14 kDa

Observed band size: 14 kDa

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