Cusabio
SKU(재고 관리 코드):CSB-PA010289ESR1HU
HEPH Antibody
HEPH Antibody
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| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-PA010289ESR1HU |
| Short Name | HEPH Antibody |
| Antibody Type | Polyclonal Antibody |
| List Price | $299/100ul, $166/50ul |
| Species Reactivity | Human |
| Tested Applications | ELISA, WB, IHC |
| Dilution | WB:1:1000-1:5000, IHC:1:20-1:200 |
| Purification Method | Antigen affinity purification |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | Recombinant human Hephaestin protein (300-580aa) |
| Antigen Species | Human |
| Uniprot ID | Q9BQS7 |
| Target Name | HEPH |
| Clonality | Polyclonal |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/11909893/ |
| Size | 100ul |
Image 1
Western Blot
Positive WB detected in: K562 whole cell lysate(30µg), Hela whole cell lysate(30µg), MCF7 whole cell lysate(30µg)
All lanes: HEPH antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/20000 dilution
Predicted band size: 131,137,101 kDa
Observed band size: 101 kDa
Exposure time: 120s
Image 2
IHC image of CSB-PA010289ESR1HU diluted at 1:30 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody
Image 3
IHCimageofCSB-PA010289ESR1HUdilutedat1:30andstaininginparaffin-embeddedhumancolorectalcancerperformedonaLeicaBondTMsystem.Afterdewaxingandhydration,antigenretrievalwasmediatedbyhighpressureinacitratebuffer(pH6.0).Sectionwasblockedwith10%normalgoatserum30minatRT.Thenprimaryantibody(1%BSA)wasincubatedat4°Covernight.TheprimaryisdetectedbyaGoatanti-rabbitpolymerIgGlabeledbyHRPandvisualizedusing0.05%DAB. Secondaryantibodyonlycontrol: uses 1%BSA instead of primary antibody
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