Cusabio
SKU(재고 관리 코드):CSB-PA010378PA96acHU
Acetyl-HIST1H1C (K96) Antibody
Acetyl-HIST1H1C (K96) Antibody
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| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-PA010378PA96acHU |
| Short Name | Acetyl-HIST1H1C (K96) Antibody |
| Antibody Type | Polyclonal Antibody |
| List Price | $319/100ul, $175/50ul |
| Species Reactivity | Human |
| Tested Applications | ELISA, WB, ICC, IF, IP, ChIP |
| Dilution | WB:1:1000-1:5000, ICC:1:20-1:200, IF:1:50-1:200, IP:1:200-1:2000 |
| Purification Method | Antigen affinity purification |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | Peptide sequence around site of Acetyl-Lys (96) derived from human Histone H1.2 |
| Antigen Species | Human |
| Uniprot ID | P16403 |
| Target Name | HIST1H1C |
| Clonality | Polyclonal |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12782899/ |
| Size | 100ul |
Image 1
Western Blot
Detected samples: Hela whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, Jurkat whole cell lysate; Untreated (-) or treated (+) with 30mM sodium butyrate for 4h
All lanes: HIST1H1C antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 22 kDa
Observed band size: 22 kDa
Image 2
Immunocytochemistry analysis of Hela cells using CSB-PA010378PA96acHU at dilution of 1:100
Image 3
Immunofluorescent analysis of Hela cells (sodium butyrate, 30 mM, 4h) using CSB-PA010378PA96acHU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Image 4
Chromatin Immunoprecipitation Hela (4*10<sup>6</sup>, treated with 30mM sodium butyrate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 8µg anti-HIST1H1C (CSB-PA010378PA96acHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
Image 5
Immunoprecipitating HIST1H1C in Hela whole cell lysate (treated with 30mM sodium butyrate for 4h)
Lane 1: Rabbit control IgG instead of CSB-PA010378PA96acHU in Hela whole cell lysate (treated with 30mM sodium butyrate for 4h). For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA010378PA96acHU (5µg) + Hela whole cell lysate (treated with 30mM sodium butyrate for 4h) (500µg)
Lane 3: Hela whole cell lysate (treated with 30mM sodium butyrate for 4h) (20µg)
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