Cusabio
SKU(재고 관리 코드):CSB-PA010418OA04acHU
Acetyl-HIST1H3A (K4) Antibody
Acetyl-HIST1H3A (K4) Antibody
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| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-PA010418OA04acHU |
| Short Name | Acetyl-HIST1H3A (K4) Antibody |
| Antibody Type | Polyclonal Antibody |
| List Price | $319/100ul, $175/50ul |
| Species Reactivity | Human |
| Tested Applications | ELISA, WB, IF, IP, ChIP |
| Dilution | WB:1:200-1:2000, IF:1:1-1:10, IP:1:200-1:2000 |
| Purification Method | Antigen affinity purification |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | Peptide sequence around site of Acetyl-Lys (4) derived from human Histone H3.1 |
| Antigen Species | Human |
| Uniprot ID | P68431 |
| Target Name | HIST1H3A |
| Clonality | Polyclonal |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12784025/ |
| Size | 50ul |
Image 1
Immunofluorescence staining of Hela cells with CSB-PA010418OA04acHU at 1:7.5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Image 2
Chromatin Immunoprecipitation Hela (4*10<sup>6</sup>, treated with 30mM sodium butyrate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-HIST1H3A (CSB-PA010418OA04acHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
Image 3
Western Blot
Positive WB detected in: Jurkat whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate (treated by 30mM sodium butyrate for 4h)
All lanes: HIST1H3A antibody at 0.935µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 16 kDa
Observed band size: 16 kDa
Image 4
Immunoprecipitating HIST1H3A in HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h)
Lane 1: Rabbit control IgG instead of CSB-PA010418OA04acHU in HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h). For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA010418OA04acHU (5µg) + HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h) (500µg)
Lane 3: HepG2 whole cell lysate (treated with 30mM sodium butyrate for 4h) (20µg)
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