Cusabio
SKU(재고 관리 코드):CSB-PA010418OA04nacHU
HIST1H3A (Ab-4) Antibody
HIST1H3A (Ab-4) Antibody
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| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-PA010418OA04nacHU |
| Short Name | HIST1H3A (Ab-4) Antibody |
| Antibody Type | Polyclonal Antibody |
| List Price | $319/100ul, $175/50ul |
| Species Reactivity | Human, Mouse |
| Tested Applications | ELISA, WB, IHC, IF, ChIP |
| Dilution | WB:1:50-1:500, IHC:1:20-1:200, IF:1:1-1:10 |
| Purification Method | Antigen affinity purification |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | Peptide sequence around site of Lys (4) derived from human Histone H3.1 |
| Antigen Species | Human |
| Uniprot ID | P68431 |
| Target Name | HIST1H3A |
| Clonality | Polyclonal |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12784026/ |
| Size | 50ul |
Image 1
Western Blot
Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate, Mouse liver tissue, Mouse kidney tissue
All lanes: HIST1H3A antibody at 2.48µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 16 kDa
Observed band size: 16 kDa
Image 2
IHC image of CSB-PA010418OA04nacHU diluted at 1:100 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Image 3
IHC image of CSB-PA010418OA04nacHU diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Image 4
Immunofluorescence staining of Hela cells with CSB-PA010418OA04nacHU at 1:4, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Image 5
Chromatin Immunoprecipitation Hela (4*10<sup>6</sup>) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-HIST1H3A (CSB-PA010418OA04nacHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
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