Cusabio
SKU(재고 관리 코드):CSB-PA010418OA36acHU
Acetyl-HIST1H3A (K36) Antibody
Acetyl-HIST1H3A (K36) Antibody
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| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-PA010418OA36acHU |
| Short Name | Acetyl-HIST1H3A (K36) Antibody |
| Antibody Type | Polyclonal Antibody |
| List Price | $319/100ul, $175/50ul |
| Species Reactivity | Human |
| Tested Applications | ELISA, WB, IF, ChIP |
| Dilution | WB:1:500-1:2000, IF:1:20-1:200 |
| Purification Method | Antigen affinity purification |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | Peptide sequence around site of Acetyl-Lys (36) derived from human Histone H3.1 |
| Antigen Species | Human |
| Uniprot ID | P68431 |
| Target Name | HIST1H3A |
| Clonality | Polyclonal |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12784029/ |
| Size | 100ul |
Image 1
Western Blot
Detected samples: Jurkat whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate; Untreated (-) or treated (+) with 30mM sodium butyrate for 4h
All lanes: HIST1H3A antibody at 1:500
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 16 kDa
Observed band size: 16 kDa
Image 2
Immunofluorescence staining of Hela cells with CSB-PA010418OA36acHU at 1:37.5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Image 3
Chromatin Immunoprecipitation Hela (4*10<sup>6</sup>, treated with 30mM sodium butyrate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-HIST1H3A (CSB-PA010418OA36acHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
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