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Cusabio

SKU(재고 관리 코드):CSB-PA010418PA09acHU

Acetyl-HIST1H3A (K9) Antibody

Acetyl-HIST1H3A (K9) Antibody

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통합 견적 요청
Size
Release Date 2022-04-01
Product Code CSB-PA010418PA09acHU
Short Name Acetyl-HIST1H3A (K9) Antibody
Antibody Type Polyclonal Antibody
List Price $319/100ul, $175/50ul
Species Reactivity Human
Tested Applications ELISA, WB, ICC, ChIP
Dilution WB:1:500-1:2000, ICC:1:20-1:200
Purification Method Antigen affinity purification
Buffer Formulation 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4.
Antigen Peptide sequence around site of Acetyl-Lys (9) derived from human Histone H3.1
Antigen Species Human
Uniprot ID P68431
Target Name HIST1H3A
Clonality Polyclonal
Host Species Rabbit
Isotype IgG
Conjugation Non-conjugated
Form Liquid
Storage Condition Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
TDS URL https://www.cusabio.com/datasheet/12782756/
Size 100ul

Image 1

Western Blot

Detected samples: Hela whole cell lysate, Jurkat whole cell lysate, 293 whole cell lysate, HepG2 whole cell lysate; Untreated (-) or treated (+) with 30mM sodium butyrate for 4h

All lanes: HIST1H3A antibody at 1:500

Secondary

Goat polyclonal to rabbit IgG at 1/40000 dilution

Predicted band size: 16 kDa

Observed band size: 16 kDa

Image 2

Immunocytochemistry analysis of Hela cells using CSB-PA010418PA09acHU at dilution of 1:100

Image 3

Chromatin Immunoprecipitation Hela (4*10<sup>6</sup>, treated with 30mM sodium butyrate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5µg anti-HIST1H3A (CSB-PA010418PA09acHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.

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