Cusabio
SKU(재고 관리 코드):CSB-PA010429PA08nacHU
HIST1H4A (Ab-8) Antibody
HIST1H4A (Ab-8) Antibody
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| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-PA010429PA08nacHU |
| Short Name | HIST1H4A (Ab-8) Antibody |
| Antibody Type | Polyclonal Antibody |
| List Price | $319/100ul, $175/50ul |
| Species Reactivity | Human, Mouse, Rat |
| Tested Applications | ELISA, WB, IHC, IF, IP, ChIP |
| Dilution | WB:1:100-1:1000, IHC:1:20-1:200, IF:1:50-1:200, IP:1:200-1:2000 |
| Purification Method | Antigen affinity purification |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | Peptide sequence around site of Lys (8) derived from human Histone H4 |
| Antigen Species | Human |
| Uniprot ID | P62805 |
| Target Name | HIST1H4A |
| Clonality | Polyclonal |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12782808/ |
| Size | 50ul |
Image 1
Western Blot
Positive WB detected in: Rat liver tissue
All lanes: HIST1H4A antibody at 2µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 12 kDa
Observed band size: 12 kDa
Image 2
Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA010429PA08nacHU at dilution of 1:100
Image 3
Immunohistochemistry of paraffin-embedded human lung cancer using CSB-PA010429PA08nacHU at dilution of 1:100
Image 4
Immunofluorescent analysis of Hela cells using CSB-PA010429PA08nacHU at dilution of 1:100 and Cy3-congugated Goat Anti-Rabbit IgG
Image 5
Chromatin Immunoprecipitation Hela (4*10<sup>6</sup>) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 8µg anti-HIST1H4A (CSB-PA010429PA08nacHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.
Image 6
Immunoprecipitating HIST1H4A in NIH/3T3 whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA010429PA08nacHU in NIH/3T3 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA010429PA08nacHU (5µg) + NIH/3T3 whole cell lysate (500µg)
Lane 3: NIH/3T3 whole cell lysate (20µg)
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