Cusabio
SKU(재고 관리 코드):CSB-PA012091LA01HU
KCNQ3 Antibody
KCNQ3 Antibody
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| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-PA012091LA01HU |
| Short Name | KCNQ3 Antibody |
| Antibody Type | Polyclonal Antibody |
| List Price | $299/100ul, $166/50ul |
| Species Reactivity | Human, Mouse, Rat |
| Tested Applications | ELISA, WB, IF |
| Dilution | WB:1:500-1:5000, IF:1:50-1:200 |
| Purification Method | Antigen affinity purification |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | Recombinant human Potassium voltage-gated channel subfamily KQT member 3 protein (763-872aa) |
| Antigen Species | Human |
| Uniprot ID | O43525 |
| Target Name | KCNQ3 |
| Clonality | Polyclonal |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12781767/ |
| Size | 50ul |
Image 1
Western Blot
Positive WB detected in: K562 whole cell lysate(30µg), THP-1 whole cell lysate(30µg), JK whole cell lysate(30µg), HepG2 whole cell lysate(30µg), A549 whole cell lysate(30µg), Mouse Brain tissue lysate(30µg),Rat Brain tissue lysate(30µg)
All lanes: KCNQ3 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/20000 dilution
Predicted band size: 97,85,90,100 kDa
Observed band size: 100 kDa
Exposure time:120s
Image 2
Immunofluorescence staining of HepG2 cell with CSB-PA012091LA01HU at 1:10, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Image 3
Immunofluorescence staining of HepG2 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Image 4
Immunofluorescence staining of SH-SY5Y cell with CSB-PA012091LA01HU at 1:10, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Image 5
Immunofluorescence staining of SH-SY5Y cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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