Cusabio
SKU(재고 관리 코드):CSB-PA015988LA01HU
NPHS1 Antibody
NPHS1 Antibody
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| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-PA015988LA01HU |
| Short Name | NPHS1 Antibody |
| Antibody Type | Polyclonal Antibody |
| List Price | $299/100ug, $166/50ug |
| Species Reactivity | Human |
| Tested Applications | ELISA, WB, IHC |
| Dilution | WB:1:1000-1:3000, IHC:1:100-1:300 |
| Purification Method | Antigen affinity purification |
| Buffer Formulation | PBS, 50% Glycerol, 0.5% BSA, 0.02% Sodium Azide, pH 7.4. |
| Antigen | A synthesized peptide derived from human Nephrin |
| Antigen Species | Human |
| Uniprot ID | O60500 |
| Target Name | NPHS1 |
| Clonality | Polyclonal |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/1090438/ |
| Size | 50ug |
Image 1
Positive WB detected in: JK whole cell lysate30µg), U251 whole cell lysate(30µg), MCF7 whole cell lysate(30µg), 293T whole cell lysate(30µg)
All lanes: NPHS1 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 135,131 kDa
Observed band size: 135 kDa
Exposure time：120s
Image 2
IHC image of CSB-PA015988LA01HU diluted at 1:100 and staining in paraffin-embedded human colorectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Secondary antibody only control: uses 1% BSA instead of primary antibody
Image 3
IHC image of CSB-PA015988LA01HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Secondary antibody only control: uses 1% BSA instead of primary antibody
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