• NUP98 Antibody
  • NUP98 Antibody
  • NUP98 Antibody
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Cusabio

CSB-PA016209LA01HU

NUP98 Antibody

NUP98 Antibody

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이 제품 견적 요청

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통합 견적 요청
Size
Release Date 2022-04-01
Product Code CSB-PA016209LA01HU
Short Name NUP98 Antibody
Antibody Type Polyclonal Antibody
List Price $299/100ug, $166/50ug
Species Reactivity Human
Tested Applications ELISA, WB, IF, IP
Dilution WB:1:500-1:5000, IF:1:50-1:200, IP:1:200-1:2000
Purification Method Protein G purification
Buffer Formulation 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4.
Antigen Recombinant human Nuclear pore complex protein Nup98-Nup96 protein (965-1110aa)
Antigen Species Human
Uniprot ID P52948
Target Name NUP98
Clonality Polyclonal
Host Species Rabbit
Isotype IgG
Conjugation Non-conjugated
Form Liquid
Storage Condition Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
TDS URL https://www.cusabio.com/datasheet/12782614/
Size 50ug

Image 1

Western Blot

Positive WB detected in: MCF-7 whole cell lysate, Hela whole cell lysate

All lanes: NUP98 antibody at 3.2ug/ml

Secondary

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 198, 188, 98, 97, 196, 187 kDa

Observed band size: 105 kDa

Image 2

Immunofluorescence staining of A549 cells with CSB-PA016209LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Image 3

Immunoprecipitating NUP98 in Jurkat whole cell lysate

Lane 1: Rabbit control IgG instead of CSB-PA016209LA01HU in Jurkat whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)

Lane 2: CSB-PA016209LA01HU (8ug) + Jurkat whole cell lysate (500ug)

Lane 3: Jurkat whole cell lysate (20ug)

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