Cusabio
SKU(재고 관리 코드):CSB-PA022517LA01HU
SPCS3 Antibody
SPCS3 Antibody
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| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-PA022517LA01HU |
| Short Name | SPCS3 Antibody |
| Antibody Type | Polyclonal Antibody |
| List Price | $600/100ul |
| Species Reactivity | Human, Mouse |
| Tested Applications | ELISA, WB, IHC |
| Dilution | WB:1:500-1:2000, IHC:1:20-1:200 |
| Purification Method | Antigen affinity purification |
| Buffer Formulation | PBS, 0.02% Sodium Azide, 50% Glycerol, pH 7.4. |
| Antigen | Recombinant human Signal peptidase complex subunit 3 protein |
| Antigen Species | Human |
| Uniprot ID | P61009 |
| Target Name | SPCS3 |
| Clonality | Polyclonal |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/11112225/ |
| Size | 100ul |
Image 1
Immunohistochemistry of paraffin-embedded human liver tissue using CSB-PA022517LA01HU at dilution of 1:100
Image 2
Immunohistochemistry of paraffin-embedded human placenta tissue using CSB-PA022517LA01HU at dilution of 1:100
Image 3
Western Blot
Positive WB detected in: 293T whole cell lysate, Hela whole cell lysate, K562 whole cell lysate, A431 whole cell lysate, JK whole cell lysate, Mouset small intestinetissue lysate
All lanes: SPCS3 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 21 kDa
Observed band size: 21kDa
Image 4
IHC image of CSB-PA022517LA01HU diluted at 1:150 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Image 5
IHC image of CSB-PA022517LA01HU diluted at 1:150 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Secondary antibody only control: uses 1% BSA instead of primary antibody
Image 6
IHC image of CSB-PA022517LA01HU diluted at 1:150 and staining in paraffin-embedded mouse liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Secondary antibody only control: uses 1% BSA instead of primary antibody
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