Cusabio
SKU(재고 관리 코드):CSB-PA07889A0Rb
TP53 Antibody
TP53 Antibody
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| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-PA07889A0Rb |
| Short Name | TP53 Antibody |
| Antibody Type | Polyclonal Antibody |
| List Price | $299/100ug, $166/50ug |
| Species Reactivity | Human, Rat |
| Tested Applications | ELISA, WB, IF |
| Dilution | WB:1:500-1:1000, IF:1:20-1:100 |
| Purification Method | Protein G purification |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | Recombinant human Cellular tumor antigen p53 protein (2-393aa) |
| Antigen Species | Human |
| Uniprot ID | P04637 |
| Target Name | TP53 |
| Clonality | Polyclonal |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/11106204/ |
| Size | 100ug |
Image 1
Western Blot
Positive WB detected in: MCF7 whole cell lysate(20µg), SY5Y whole cell lysate(20µg), A549 whole cell lysate(20µg), HepG2 whole cell lysate(20µg), Rat colorectal tissue lysate(20µg)
All lanes: TP53 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/20000 dilution
Predicted band size: 44,38,39,40,34,35,30,24,25 kDa
Observed band size: 53 kDa
Exposure time: 120s
Image 2
Immunofluorescence staining of A549 cell with CSB-PA07889A0Rb at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Image 3
Immunofluorescence staining of A549 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Image 4
Immunofluorescence staining of MCF-7 cell with CSB-PA07889A0Rb at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Image 5
Immunofluorescence staining of MCF-7 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Image 6
Immunofluorescence staining of U251 cell with CSB-PA07889A0Rb at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Image 7
Immunofluorescence staining of U251 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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