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Cusabio

SKU(재고 관리 코드):CSB-PA07939A0Rb

TNNI3 Antibody

TNNI3 Antibody

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통합 견적 요청
Size
Release Date 2022-04-01
Product Code CSB-PA07939A0Rb
Short Name TNNI3 Antibody
Antibody Type Polyclonal Antibody
List Price $299/100ug, $166/50ug
Species Reactivity Human, Rat
Tested Applications ELISA, IHC, IF, IP
Dilution IHC:1:20-1:200, IF:1:200-1:500, IP:1:200-1:2000
Purification Method Protein G purification
Buffer Formulation 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4.
Antigen Recombinant human Troponin I, cardiac muscle protein (2-210aa)
Antigen Species Human
Uniprot ID P19429
Target Name TNNI3
Clonality Polyclonal
Host Species Rabbit
Isotype IgG
Conjugation Non-conjugated
Form Liquid
Storage Condition Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
TDS URL https://www.cusabio.com/datasheet/12927063/
Size 50ug

Image 1

Immunofluorescence staining of Hela cells with CSB-PA07939A0Rb at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Image 2

Immunohistochemistry of paraffin-embedded human heart tissue using CSB-PA07939A0Rb at dilution of 1:100

Image 3

Immunoprecipitating TNNI3 in Rat heart tissue

Lane 1: Rabbit control IgG instead of CSB-PA07939A0Rb in Rat heart tissue. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/5000)

Lane 2: CSB-PA07939A0Rb (8µg) + Rat heart tissue (500µg)

Lane 3: Rat heart tissue (20µg)

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