• TUBA4A Antibody
  • TUBA4A Antibody
  • TUBA4A Antibody
  • TUBA4A Antibody
  • TUBA4A Antibody
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Cusabio

CSB-PA09499A0Rb

TUBA4A Antibody

TUBA4A Antibody

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이 제품 견적 요청

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통합 견적 요청
Size
Release Date 2022-04-01
Product Code CSB-PA09499A0Rb
Short Name TUBA4A Antibody
Antibody Type Polyclonal Antibody
List Price $299/100ug, $166/50ug
Species Reactivity Human, Mouse
Tested Applications ELISA, WB, IHC, IF, IP
Dilution WB:1:1000-1:5000, IHC:1:20-1:200, IF:1:50-1:200, IP:1:200-1:2000
Purification Method Protein G purification
Buffer Formulation 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4.
Antigen Recombinant human Tubulin alpha-4A chain protein (20-183aa)
Antigen Species Human
Uniprot ID P68366
Target Name TUBA4A
Clonality Polyclonal
Host Species Rabbit
Isotype IgG
Conjugation Non-conjugated
Form Liquid
Storage Condition Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
TDS URL https://www.cusabio.com/datasheet/12548431/
Size 100ug

Image 1

Western Blot

Positive WB detected in: NIH/3T3 whole cell lysate, K562 whole cell lysate, Mouse brain tissue

All lanes: TUBA4A antibody at 3ug/ml

Secondary

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 50, 49 kDa

Observed band size: 50, 24 kDa

Image 2

Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA09499A0Rb at dilution of 1:100

Image 3

Immunohistochemistry of paraffin-embedded human gastric cancer using CSB-PA09499A0Rb at dilution of 1:100

Image 4

Immunofluorescent analysis of HepG2 cells using CSB-PA09499A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Image 5

Immunoprecipitating TUBA4A in K562 whole cell lysate

Lane 1: Rabbit control IgG instead of (1ug) instead of CSB-PA09499A0Rb in K562 whole cell lysate.
For western blotting, a HRP-conjugated light chain specific antibody was used as the Secondary antibody (1/50000)

Lane 2: CSB-PA09499A0Rb (4ug) + K562 whole cell lysate (500ug)

Lane 3: K562 whole cell lysate (20ug)

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