Cusabio
SKU(재고 관리 코드):CSB-PA10629A0Rb
KRT18 Antibody
KRT18 Antibody
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| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-PA10629A0Rb |
| Short Name | KRT18 Antibody |
| Antibody Type | Polyclonal Antibody |
| List Price | $299/100ug, $166/50ug |
| Species Reactivity | Human, Zebrafish |
| Tested Applications | ELISA, WB, IHC, IF |
| Dilution | WB:1:500-1:5000, IHC:1:100-1:1000, IF:1:50-1:500 |
| Purification Method | Protein G purification |
| Buffer Formulation | 0.1mol/L NaCl, 15mmol/L NaN3, pH 7.2. |
| Antigen | Recombinant human Keratin, type I cytoskeletal 18 protein (102-396aa) |
| Antigen Species | Human |
| Uniprot ID | P05783 |
| Target Name | KRT18 |
| Clonality | Polyclonal |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/1081387/ |
| Size | 50ug |
Image 1
Western blot
All lanes: KRT18 antibody at 2µg/ml
Lane 1: K562 whole cell lysate
Lane 2: HepG2 whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 49 kDa
Observed band size: 49 kDa
Image 2
Western blot
All lanes: KRT18 antibody at 12µg/ml
Lane 1: A431 whole cell lysate
Lane 2: Hela whole cell lysate
Lane 3: Jurkat whole cell lysate
Lane 4: Zebrafish lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 49 kDa
Observed band size: 49 kDa
Image 3
Western Blot
Positive WB detected in: K562 whole cell lysate, Hela whole cell lysate
All lanes: KRT18 antibody at 3µg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 49 kDa
Observed band size: 49 kDa
Image 4
IHC image of CSB-PA10629A0Rb diluted at 1:800 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Image 5
Immunofluorescent analysis of HepG2 cells using CSB-PA10629A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Image 6
Immunocytochemistry analysis of human placenta tissue using CSB-PA10629A0Rb at dilution of 1:100
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