Cusabio
SKU(재고 관리 코드):CSB-PA16889A0Rb
MSH2 Antibody
MSH2 Antibody
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| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-PA16889A0Rb |
| Short Name | MSH2 Antibody |
| Antibody Type | Polyclonal Antibody |
| List Price | $299/100ug, $166/50ug |
| Species Reactivity | Human, Mouse |
| Tested Applications | ELISA, WB, IHC, IP |
| Dilution | WB:1:500-1:5000, IHC:1:200-1:500, IP:1:200-1:2000 |
| Purification Method | Protein G purification |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | Recombinant human DNA mismatch repair protein Msh2 protein (2-254aa) |
| Antigen Species | Human |
| Uniprot ID | P43246 |
| Target Name | MSH2 |
| Clonality | Polyclonal |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12555464/ |
| Size | 50ug |
Image 1
Western Blot
Positive WB detected in: Hela whole cell lysate, NIH/3T3 whole cell lysate, A549 whole cell lysate, HEK293 whole cell lysate
All lanes: MSH2 antibody at 2ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 105, 98 kDa
Observed band size: 105, 98 kDa
Image 2
IHC image of CSB-PA16889A0Rb diluted at 1:400 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Image 3
Immunoprecipitating MSH2 in 293 whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA16889A0Rb in 293 whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA16889A0Rb (6ug) + 293 whole cell lysate (1mg)
Lane 3: 293 whole cell lysate (20ug)
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