Cusabio
SKU(재고 관리 코드):CSB-PA17859A0Rb
KAT8 Antibody
KAT8 Antibody
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| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-PA17859A0Rb |
| Short Name | KAT8 Antibody |
| Antibody Type | Polyclonal Antibody |
| List Price | $600/100ul |
| Species Reactivity | Human, Mouse, Rat |
| Tested Applications | ELISA, WB, IF |
| Dilution | WB:1:500-1:5000, IF:1:10-1:100 |
| Purification Method | Antigen affinity purification |
| Buffer Formulation | PBS, 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Antigen | Recombinant Human Histone acetyltransferase KAT8 protein (120-467AA) |
| Antigen Species | Human |
| Uniprot ID | Q9H7Z6 |
| Target Name | KAT8 |
| Clonality | Polyclonal |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12552089/ |
| Size | 100ul |
Image 1
Western Blot
Positive WB detected in: Hela whole cell lysate(30µg), MCF7 whole cell lysate(30µg), THP-1 whole cell lysate(30µg), Raji whole cell lysate(30µg), SY5Y whole cell lysate(30µg), A2780 whole cell lysate(30µg),HepG2 whole cell lysate(30µg),Mouse Liver tissue lysate(30µg),Rat Liver tissue lysate(30µg)
All lanes: KAT8 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 53 kDa
Observed band size: 53 kDa
Exposure time: 10s
Image 2
Immunofluorescence staining of HepG2 cell with CSB-PA17859A0Rb at 1:15, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and and permeated by 0.2% TritonX-100 for 15 min. Then 10% normal goat serum to block non-specific protein-protein interactions . The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Image 3
Immunofluorescence staining of MCF-7 cell with CSB-PA17859A0Rb at 1:15, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and and permeated by 0.2% TritonX-100 for 15 min. Then 10% normal goat serum to block non-specific protein-protein interactions . The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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