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Cusabio

SKU(재고 관리 코드):CSB-PA623101LA01HU

IMMT Antibody

IMMT Antibody

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통합 견적 요청
Size
Release Date 2022-04-01
Product Code CSB-PA623101LA01HU
Short Name IMMT Antibody
Antibody Type Polyclonal Antibody
List Price $299/100ug, $166/50ug
Species Reactivity Human
Tested Applications ELISA, WB, IHC, IF, IP
Dilution WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200, IP:1:200-1:2000
Purification Method Protein G purification
Buffer Formulation 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4.
Antigen Recombinant human MICOS complex subunit MIC60 protein (76-215aa)
Antigen Species Human
Uniprot ID Q16891
Target Name IMMT
Clonality Polyclonal
Host Species Rabbit
Isotype IgG
Conjugation Non-conjugated
Form Liquid
Storage Condition Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.
TDS URL https://www.cusabio.com/datasheet/12554370/
Size 50ug

Image 1

Western Blot

Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate

All lanes: IMMT antibody at 3.5ug/ml

Secondary

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 84, 83, 81 kDa

Observed band size: 84 kDa

Image 2

Immunohistochemistry of paraffin-embedded human glioma using CSB-PA623101LA01HU at dilution of 1:100

Image 3

Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA623101LA01HU at dilution of 1:100

Image 4

Immunofluorescent analysis of HepG2 cells using CSB-PA623101LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Image 5

Immunoprecipitating IMMT in HepG2 whole cell lysate

Lane 1: Rabbit control IgG instead of CSB-PA623101LA01HU in HepG2 whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)

Lane 2: CSB-PA623101LA01HU (8ug) + HepG2 whole cell lysate (500ug)

Lane 3: HepG2 whole cell lysate (10ug)

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