Cusabio
SKU(재고 관리 코드):CSB-PA803912ZA01MO
Clec4g Antibody
Clec4g Antibody
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| Release Date | 2023-12-13 |
|---|---|
| Product Code | CSB-PA803912ZA01MO |
| Short Name | Clec4g Antibody |
| Antibody Type | Polyclonal Antibody |
| List Price | $299/100ul, $166/50ul |
| Species Reactivity | Mus musculus |
| Tested Applications | ELISA, WB, IHC, IF |
| Dilution | WB:1:500-1:5000, IHC:1:50-1:200, IF:1:30-1:100 |
| Purification Method | Antigen affinity purification |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | Recombinant mouse Clec4g protein (52-294aa) |
| Antigen Species | Mouse |
| Uniprot ID | Q8BNX1 |
| Target Name | Clec4g |
| Clonality | Polyclonal |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12931607/ |
| Size | 100ul |
Image 1
Western Blot
Positive WB detected in: Mouse Heart tissue lysate
All lanes: Clec4g antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 34 kDa
Observed band size: 34 kDa
Image 2
Western Blot
Positive WB detected in: Mouse Heart tissue lysate
All lanes: Clec4g antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 34 kDa
Observed band size: 34 kDa
Image 3
IHC image of CSB-PA803912ZA01MO diluted at 1:66 and staining in paraffin-embedded human spleen tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Image 4
IHC image of CSB-PA803912ZA01MO diluted at 1:66 and staining in paraffin-embedded human spleen tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Image 5
Immunofluorescence staining of Hela cell with CSB-PA803912ZA01MO at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Image 6
Immunofluorescence staining of NIH/3T3 cell with CSB-PA803912ZA01MO at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Image 7
Immunofluorescence staining of Hela cell with CSB-PA803912ZA01MO at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Image 8
Immunofluorescence staining of NIH/3T3 cell with CSB-PA803912ZA01MO at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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