Cusabio
SKU(재고 관리 코드):CSB-PA871402LA01HU
TUBGCP4 Antibody
TUBGCP4 Antibody
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| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-PA871402LA01HU |
| Short Name | TUBGCP4 Antibody |
| Antibody Type | Polyclonal Antibody |
| List Price | $299/100ug, $166/50ug |
| Species Reactivity | Human |
| Tested Applications | ELISA, WB, IHC, IF, IP |
| Dilution | WB:1:500-1:5000, IHC:1:20-1:200, IF:1:200-1:500, IP:1:200-1:2000 |
| Purification Method | Protein G purification |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | Recombinant human Gamma-tubulin complex component 4 protein (203-309aa) |
| Antigen Species | Human |
| Uniprot ID | Q9UGJ1 |
| Target Name | TUBGCP4 |
| Clonality | Polyclonal |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12781472/ |
| Size | 50ug |
Image 1
Western Blot
Positive WB detected in: MCF-7 whole cell lysate
All lanes: TUBGCP4 antibody at 3ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 77 kDa
Observed band size: 77 kDa
Image 2
Immunohistochemistry of paraffin-embedded human small intestine tissue using CSB-PA871402LA01HU at dilution of 1:100
Image 3
Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA871402LA01HU at dilution of 1:100
Image 4
Immunofluorescence staining of HepG2 cells with CSB-PA871402LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Image 5
Immunoprecipitating TUBGCP4 in MCF-7 whole cell lysate
Lane 1: Rabbit control IgG instead of CSB-PA871402LA01HU in MCF-7 whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-PA871402LA01HU (8ug) + MCF-7 whole cell lysate (500ug)
Lane 3: MCF-7 whole cell lysate (10ug)
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