Cusabio
SKU(재고 관리 코드):CSB-RA000091M1m
ACTB Recombinant Monoclonal Antibody
ACTB Recombinant Monoclonal Antibody
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| Release Date | 2024-10-31 |
|---|---|
| Product Code | CSB-RA000091M1m |
| Short Name | ACTB Recombinant Monoclonal Antibody |
| Antibody Type | Recombinant Antibody |
| List Price | $350/100ul, $210/50ul |
| Species Reactivity | Human |
| Tested Applications | ELISA, WB, FC |
| Dilution | WB:1:500-1:2000, FC:1:50-1:200 |
| Purification Method | Affinity chromatography |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | Recombinant Human ACTB protein |
| Antigen Species | Human |
| Uniprot ID | P60709 |
| Target Name | ACTB |
| Clonality | Monoclonal |
| Clone ID | 1D12E12B5 |
| Host Species | Mouse |
| Isotype | IgG2b |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12938149/ |
| Size | 100ul |
Image 1
Western Blot
Positive WB detected in:U251 whole cell lysate (20µg) , Hela whole cell lysate (20µg) , HepG2 whole cell lysate (20µg) , 293T whole cell lysate (20µg) , A549 whole cell lysate (20µg) , MCF7 whole cell lysate (20µg)
All lanes: ACTB antibody at 1:1000
Secondary
Goat polyclonal to mouse IgG at 1/40000 dilution
Predicted band size:42 kDa
Observed band size:42 kDa
Exposure time:20s
Image 2
Overlay Peak curve showing Hela cells stained with CSB-RA000091M1m (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10<sup>6</sup>cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Antimouse IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was mouse IgG (1ug/1*10<sup>6</sup>cells) used under the same conditions. Acquisition of >10,000 events was performed.
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