GAPDH Recombinant Monoclonal Antibody

Image 1

Western Blot

Positive WB detected in: HepG2 whole cell lysate,U87 whole cell lysate,JK whole cell lysate

All lanes: GAPDH antibody at 1:500

Secondary

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 36 kDa

Observed band size: 36 kDa

Image 2

IHC image of CSB-RA009232MA1HU diluted at 1:50 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.

Image 3

Immunofluorescence staining of HepG2 cell with CSB-RA009232MA1HU at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) .

Image 4

Overlay Peak curve showing Hela cells surface stained with CSB-RA009232MA1HU (red line) at 1:50. The cells were fixed in 4% formaldehyde (15min) and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*10⁶cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.