Cusabio
SKU(재고 관리 코드):CSB-RA024077MA1HU
TP53 Recombinant Monoclonal Antibody
TP53 Recombinant Monoclonal Antibody
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| Release Date | 2023-07-06 |
|---|---|
| Product Code | CSB-RA024077MA1HU |
| Short Name | TP53 Recombinant Monoclonal Antibody |
| Antibody Type | Recombinant Antibody |
| List Price | $350/100ul, $210/50ul |
| Species Reactivity | Human |
| Tested Applications | ELISA, WB, IF, FC, SPR |
| Dilution | ELISA:1:1000-1:10000, WB:1:1000-1:5000, IF:1:20-1:200, FC:1:20-1:200 |
| Purification Method | Affinity chromatography |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | A synthesized peptide derived from human TP53 |
| Antigen Species | Human |
| Uniprot ID | P04637 |
| Target Name | TP53 |
| Clonality | Monoclonal |
| Clone ID | 16D9 |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12935075/ |
| Size | 50ul |
Image 1
Western Blot
Positive WB detected in: HT-29 whole cell lysate
All lanes: TP53 antibody at 1:500
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 43 kDa
Observed band size: 43 kDa
Image 2
Immunofluorescence staining of MCF-7 cell with CSB-RA024077MA1HU at 1:60, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) .
Image 3
Immunofluorescence staining of Hela cell with CSB-RA024077MA1HU at 1:60, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) .
Image 4
Overlay Peak curve showing Hela cells surface stained with CSB-RA024077MA1HU (red line) at 1:100. The cells were fixed in 4% formaldehyde (15min) and permeated by 0.2% TritonX-100 for 10min.Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*10<sup>6</sup>cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*10<sup>6</sup>cells) used under the same conditions. Acquisition of >10,000 events was performed.
Image 5
The Binding Activity of Human TP53 with Anti-TP53 Recombinant Antibody
Activity: Measured by its binding ability in a functional ELISA. Immobilized Human TP53 (CSB-EP024077HUa0) at 2 μg/mL can bind Anti-TP53 recombinant antibody. TheEC50 is 0.5288-0.5982 ng/mL.
Image 6
The Binding Activity of Human TP53 with Anti-TP53 Recombinant Antibody
Activity：TP53 Recombinant Monoclonal Antibody captured on Protein A Chip can bind Recombinant Human TP53 (CSB-EP024077HUa0) with an affinity constant of 56.9 pM as detected by MetaSPR Assay (WeSPRTM 200).
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