Cusabio
SKU(재고 관리 코드):CSB-RA026293MA1HU
YWHAZ Recombinant Monoclonal Antibody
YWHAZ Recombinant Monoclonal Antibody
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| Release Date | 2025-07-11 |
|---|---|
| Product Code | CSB-RA026293MA1HU |
| Short Name | YWHAZ Recombinant Monoclonal Antibody |
| Antibody Type | Recombinant Antibody |
| List Price | $350/100ul, $210/50ul |
| Species Reactivity | Human, Mouse, Rat |
| Tested Applications | ELISA, WB, IF, FC |
| Dilution | WB:1:500-1:2000, IF:1:50-1:200, FC:1:50-1:200 |
| Purification Method | Affinity chromatography |
| Buffer Formulation | 0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4. |
| Antigen | Recombinant Human YWHAZ protein |
| Antigen Species | Human |
| Uniprot ID | P63104 |
| Target Name | YWHAZ |
| Clonality | Monoclonal |
| Clone ID | 19G7E9 |
| Host Species | Human |
| Isotype | IgG1 |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12942675/ |
| Size | 100ul |
Image 1
Western Blot
Positive WB detected in: HEK293T whole cell lysate(20µg) , SH-SY5Y whole cell lysate(20µg) , Jurkat whole cell lysate(20µg) , NIH/3T3 whole cell lysate(20µg) , A549 whole cell lysate(20µg) , K562 whole cell lysate(20µg) , PC-3 whole cell lysate(20µg) , MCF-7 whole cell lysate(20µg) , HeLa whole cell lysate(20µg)
All lanes: YWHAZ antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/40000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDa
Exposure time：30s
Image 2
Western Blot
Positive WB detected in: Mouse brain tissue lysate(20µg) , Rat brain tissue lysate(20µg) , Mouse uterus tissue lysate(20µg) , Rat uterus tissue lysate(20µg)
All lanes: YWHAZ antibody at 1:1000
Secondary
Goat polyclonal to human IgG at 1/40000 dilution
Predicted band size: 27.7 kDa
Observed band size: 28 kDa
Exposure time: 2min
Image 3
Immunofluorescence staining of Hela cell with CSB-RA026293MA1HU at 1:30 counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Human IgG(H+L) .
Image 4
Immunofluorescence staining of MCF-7 cell with CSB-RA026293MA1HU at 1:30 counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Human IgG(H+L) .
Image 5
Overlay Peak curve showing A431 cells stained with CSB-RA026293MA1HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10<sup>6</sup>cells) for 45min at 4℃. The secondary antibody used was Fluorescein (FITC) AffiniPure Goat Anti-Human IgG, Fcγ fragment specific at 1:200 dilution for 35min at 4℃.Control antibody (green line) was human IgG1 (1ug/1*10<sup>6</sup>cells) used under the same conditions. Acquisition of >10,000 events was performed.
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