Cusabio
SKU(재고 관리 코드):CSB-RA292372A0HU
CD44 Recombinant Monoclonal Antibody
CD44 Recombinant Monoclonal Antibody
가격·납기 및 기술자료를 확인해 드립니다.
제품 규격과 수량을 선택한 후 견적을 요청해 주세요. 대체품 검토와 SDS·COA·데이터시트 요청도 함께 접수할 수 있습니다.
이 제품 견적 요청대표번호 070-8028-3500 · info@rndmate.com
| Release Date | 2023-07-06 |
|---|---|
| Product Code | CSB-RA292372A0HU |
| Short Name | CD44 Recombinant Monoclonal Antibody |
| Antibody Type | Recombinant Antibody |
| List Price | $350/100ul, $210/50ul |
| Species Reactivity | Human |
| Tested Applications | ELISA, WB, IHC, IF, FC |
| Dilution | WB:1:500-1:2000, IHC:1:50-1:200, IF:1:50-1:200, FC:1:50-1:200 |
| Purification Method | Affinity chromatography |
| Buffer Formulation | PBS, 150mM NaCl, 50% Glycerol, 0.05% BSA, 0.02% Sodium Azide, pH 7.4. |
| Antigen | A synthesized peptide derived from human CD44 |
| Antigen Species | Human |
| Uniprot ID | P16070 |
| Target Name | CD44 |
| Clonality | Monoclonal |
| Clone ID | 19H12 |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12935025/ |
| Size | 50ul |
Image 1
Western Blot
Positive WB detected in: A549 whole cell lysate, MCF7 whole cell lysate, HepG2 whole cell lysate
All lanes: CD44 antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 81 kDa
Observed band size: 81 kDa
Image 2
IHC image of CSB-RA292372A0HU diluted at 1:50 and staining in paraffin-embedded human rectal cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.51% DAB.
Image 3
IHC image of CSB-RA292372A0HU diluted at 1:50 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.51% DAB.
Image 4
Immunofluorescence staining of Hela with CSB-RA292372A0HU at 1:25, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 515-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) .
Image 5
Overlay Peak curve showing Hela cells surface stained with CSB-RA292372A0HU (red line) at 1:50. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/1*10<sup>6</sup>cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1µg/1*10<sup>6</sup>cells) used under the same conditions. Acquisition of >10,000 events was performed.
Share
