Cusabio
SKU(재고 관리 코드):CSB-RA437652A0HU
ALCAM Antibody
ALCAM Antibody
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| Release Date | 2022-04-01 |
|---|---|
| Product Code | CSB-RA437652A0HU |
| Short Name | ALCAM Antibody |
| Antibody Type | Recombinant Antibody |
| List Price | $350/100ul, $210/50ul |
| Species Reactivity | Human, Mouse |
| Tested Applications | ELISA, WB, IHC |
| Dilution | WB:1:500-1:2000, IHC:1:50-1:200 |
| Purification Method | Affinity chromatography |
| Buffer Formulation | PBS, 150mM NaCl, 50% Glycerol, 0.05% BSA, 0.02% Sodium Azide, pH 7.4. |
| Antigen | A synthesized peptide derived from human ALCAM |
| Antigen Species | Human |
| Uniprot ID | Q13740 |
| Target Name | ALCAM |
| Clonality | Monoclonal |
| Clone ID | 21B9 |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugation | Non-conjugated |
| Form | Liquid |
| Storage Condition | Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze. |
| TDS URL | https://www.cusabio.com/datasheet/12932453/ |
| Size | 100ul |
Image 1
Western Blot
Positive WB detected in: PC3 whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, U251 whole cell lysate, A549 whole cell lysate, HEK293 whole cell lysate, Ntera-2 whole cell lysate, Mouse brain tissue
All lanes: ALCAM antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 66, 64, 16, 34 kDa
Observed band size: 100 kDa
Image 2
IHC image of CSB-RA437652A0HU diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
Image 3
IHC image of CSB-RA437652A0HU diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0) . Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
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