AGR2 Recombinant Monoclonal Antibody

Image 1

Western Blot

Positive WB detected in: COLO205 whole cell lysate(30µg) , MCF-7 whole cell lysate(30µg) , A549 whole cell lysate(30µg)

All lanes: AGR2 antibody at 1:1000

Secondary

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 20 kDa

Observed band size: 17 kDa

Exposure time: 2min

Image 2

Immunofluorescence staining of THP-1 cell with CSB-RA986192A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) .

Image 3

Overlay Peak curve showing A549 cells stained with CSB-RA986192A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/1*10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.