Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Immunogen:
The antiserum was produced against synthesized peptide derived from human Cytochrome P450 39A1. AA range:361-410
Storage:
Rabbit
Storage:
-20°C/1 year
Clonality:
Polyclonal
Isotype:
IgG
Concentration:
1 mg/ml
Observed Band:
54kD
GeneID（Human）:
51302
Human Swiss-Prot No:
Q9NYL5
Cellular localization:
Endoplasmic reticulum membrane ; Multi-pass membrane protein . Microsome membrane ; Multi-pass membrane protein .
Background:
cytochrome P450 family 39 subfamily A member 1(CYP39A1) Homo sapiens This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein is involved in the conversion of cholesterol to bile acids. Its substrates include the oxysterols 25-hydroxycholesterol, 27-hydroxycholesterol and 24-hydroxycholesterol. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013],
Immunofluorescence analysis of A549 cells, using Cytochrome P450 39A1 Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from LOVO and HUVEC cells, using Cytochrome P450 39A1 Antibody. The lane on the right is blocked with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human spleen. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).