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ELK Biotechnology
SKU(재고 관리 코드):ES2121
Cytochrome b5 Rabbit Polyclonal Antibody
Cytochrome b5 Rabbit Polyclonal Antibody
Cytochrome b5 Rabbit Polyclonal Antibody
Overview
| Product name: | Cytochrome b5 rabbit pAb |
| Alternative Names: | CYB5A; CYB5; Cytochrome b5; Microsomal cytochrome b5 type A; MCB5 |
| Applications: | WB;IHC;IF;ELISA |
| Recommended Dilutions: | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human CYB5. AA range:61-110 |
| Storage: | Rabbit |
| Storage: | -20°C/1 year |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Concentration: | 1 mg/ml |
| Observed Band: | 15kD |
| GeneID(Human): | 1528 |
| Human Swiss-Prot No: | P00167 |
| Cellular localization: | [Isoform 1]: Endoplasmic reticulum membrane; Single-pass membrane protein; Cytoplasmic side. Microsome membrane; Single-pass membrane protein; Cytoplasmic side.; [Isoform 2]: Cytoplasm. |
| Background: | The protein encoded by this gene is a membrane-bound cytochrome that reduces ferric hemoglobin (methemoglobin) to ferrous hemoglobin, which is required for stearyl-CoA-desaturase activity. Defects in this gene are a cause of type IV hereditary methemoglobinemia. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010], |
| Species Reactivity: | Human;Mouse;Rat |
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Western Blot analysis of various cells using Cytochrome b5 Polyclonal Antibody -
Immunofluorescence analysis of NIH/3T3 cells, using CYB5 Antibody. The picture on the right is blocked with the synthesized peptide. -
Western blot analysis of lysates from A549 cells, using CYB5 Antibody. The lane on the right is blocked with the synthesized peptide. -
Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
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