DAPK1; DAPK; Death-associated protein kinase 1; DAP kinase 1
Applications:
WB;IHC;IF;ELISA
Recommended Dilutions:
Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Immunogen:
The antiserum was produced against synthesized peptide derived from human DAPK1. AA range:274-323
Storage:
Rabbit
Storage:
-20°C/1 year
Clonality:
Polyclonal
Isotype:
IgG
Concentration:
1 mg/ml
Observed Band:
160kD
GeneID（Human）:
1612
Human Swiss-Prot No:
P53355
Cellular localization:
[Isoform 1]: Cytoplasm. Cytoplasm, cytoskeleton. Colocalizes with MAP1B in the microtubules and cortical actin fibers.; [Isoform 2]: Cytoplasm. Cytoplasm, cytoskeleton.
Background:
Death-associated protein kinase 1 is a positive mediator of gamma-interferon induced programmed cell death. DAPK1 encodes a structurally unique 160-kD calmodulin dependent serine-threonine kinase that carries 8 ankyrin repeats and 2 putative P-loop consensus sites. It is a tumor suppressor candidate. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013],
Western Blot analysis of MOUSE-BRAIN cells using DAPK1 Polyclonal Antibody diluted at 1:1000
Immunofluorescence analysis of COS7 cells, using DAPK1 Antibody. The picture on the right is blocked with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue, using DAPK1 Antibody. The picture on the right is blocked with the synthesized peptide.