DNA pol Theta Polyclonal Antibody

POLQ (Polymerase (DNA) Theta) is a Protein Coding gene. Among its related pathways are Platinum Pathway, Pharmacokinetics/Pharmacodynamics and DNA Double-Strand Break Repair. GO annotations related to this gene include nucleic acid binding and damaged DNA binding. An important paralog of this gene is SNRNP200. NA polymerase that promotes microhomology-mediated end-joining (MMEJ), an alternative non-homologous end-joining (NHEJ) machinery triggered in response to double-strand breaks in DNA (PubMed: 25642963, PubMed: 25643323). MMEJ is an error-prone repair pathway that produces deletions of sequences from the strand being repaired and promotes genomic rearrangements, such as telomere fusions, some of them leading to cellular transformation (PubMed: 25642963, PubMed: 25643323). POLQ acts as an inhibitor of homology-recombination repair (HR) pathway by limiting RAD51 accumulation at resected ends (PubMed: 25642963). POLQ-mediated MMEJ may be required to promote the survival of cells with a compromised HR repair pathway, thereby preventing genomic havoc by resolving unrepaired lesions (By similarity). The polymerase acts by binding directly the 2 ends of resected double-strand breaks, allowing microhomologous sequences in the overhangs to form base pairs. It then extends each strand from the base-paired region using the opposing overhang as a template. Requires partially resected DNA containing 2 to 6 base pairs of microhomology to perform MMEJ (PubMed: 25643323). The polymerase activity is highly promiscuous: unlike most polymerases, promotes extension of ssDNA and partial ssDNA (pssDNA) substrates (PubMed: 18503084, PubMed: 21050863, PubMed: 22135286). Also exhibits low-fidelity DNA synthesis, translesion synthesis and lyase activity, and it is implicated in interstrand-cross-link repair, base excision repair and DNA end-joining (PubMed: 14576298, PubMed: 18503084, PubMed: 19188258, PubMed: 24648516). Involved in somatic hypermutation of immunoglobulin genes, a process that requires the activity of DNA polymerases to ultimately introduce mutations at both A/T and C/G base pairs (By similarity).

The DNA pol Theta Polyclonal Antibody is a highly reliable and efficient tool for various molecular biology applications. This antibody is specifically designed to target and detect DNA polymerase Theta (pol θ), a crucial enzyme involved in DNA repair and recombination processes. With its exceptional specificity and sensitivity, this polyclonal antibody enables accurate and precise detection of pol θ in a wide range of samples.

The DNA pol Theta Polyclonal Antibody is produced using advanced recombinant DNA technology, ensuring high purity and quality. It has been extensively validated for its performance in various experimental techniques, including Western blotting, immunohistochemistry, and immunofluorescence. This antibody exhibits excellent affinity towards pol θ, allowing for reliable and reproducible results.

This polyclonal antibody offers numerous advantages for researchers in the field of molecular biology. Its versatility enables its use in diverse applications, such as studying DNA repair mechanisms, investigating genetic diseases, and exploring the role of pol θ in cancer development. Additionally, its high sensitivity allows for the detection of low levels of pol θ, making it suitable for analyzing samples with limited amounts of protein.

The DNA pol Theta Polyclonal Antibody is supplied in a convenient and user-friendly format, ensuring ease of use and storage. It is provided as a liquid solution, ready for immediate use without the need for additional reagents or preparations. The antibody is also available in different sizes to accommodate various experimental needs.

In summary, the DNA pol Theta Polyclonal Antibody is a reliable and efficient tool for researchers in the field of molecular biology. Its exceptional specificity, sensitivity, and versatility make it an invaluable asset for studying DNA repair and recombination processes. With its high-quality production and user-friendly format, this antibody is an ideal choice for accurate and precise detection of pol θ in various experimental techniques.