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ELK Biotechnology

ELK8730

Dog hs-cTnI(High Sensitivity Cardiac Troponin I) ELISA Kit

Dog hs-cTnI(High Sensitivity Cardiac Troponin I) ELISA Kit

Size
Sensitivity
Detection range

Dog hs-cTnI(High Sensitivity Cardiac Troponin I) ELISA Kit

Overview

Product name: Dog hs-cTnI(High Sensitivity Cardiac Troponin I) ELISA Kit
Reactivity: Dog
Alternative Names: High Sensitivity Cardiac Troponin I
Assay Type: Sandwich
Sensitivity: 4.94 pg/mL
Standard: 1000 pg/mL
Range: 15.63-1000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Cardiovascular biology
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog hs-cTnI. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog hs-cTnI. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog hs-cTnI, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog hs-cTnI in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
1000.00 2.056 1.973
500.00 1.613 1.530
250.00 1.088 1.005
125.00 0.841 0.758
62.50 0.457 0.374
31.25 0.357 0.274
15.63 0.241 0.158
0.00 0.083 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant hs-cTnI and the recovery rates were calculated by comparing the measured value to the expected amount of hs-cTnI in samples.
Matrix Recovery range Average
serum(n=5) 80-92% 86%
EDTA plasma(n=5) 92-106% 99%
Heparin plasma(n=5) 87-99% 93%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of hs-cTnI and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 95-104% 82-96% 92-105% 83-92%
EDTA plasma(n=5) 95-103% 99-106% 78-92% 83-97%
Heparin plasma(n=5) 87-95% 78-91% 97-103% 95-101%
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