1 1

ELK Biotechnology

ELK7307

Dog NT-ProBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit

Dog NT-ProBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit

Size
Sensitivity
Detection range

Dog NT-ProBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit

Overview

Product name: Dog NT-ProBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit
Reactivity: Dog
Alternative Names: NT-Pro-BNP; ; N-BNP; ANFB_HUMAN; BNP(1-32); BNP(5-29); BNP-32; Gamma-brain natriuretic peptide; nppb
Assay Type: Competitive Inhibition
Sensitivity: 24.2 pg/mL
Standard: 5000 pg/mL
Range: 78.13-5000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 2h
Research Area: Endocrinology;Cardiovascular biology;
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Dog NT-ProBNP protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog NT-ProBNP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog NT-ProBNP in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
5000.00 0.199 —
2500.00 0.386 —
1250.00 0.575 —
625.00 0.878 —
312.50 1.112 —
156.25 1.592 —
78.13 1.793 —
0.00 2.226 —

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant NT-ProBNP and the recovery rates were calculated by comparing the measured value to the expected amount of NT-ProBNP in samples.
Matrix Recovery range Average
serum(n=5) 81-96% 88%
EDTA plasma(n=5) 78-94% 86%
Heparin plasma(n=5) 90-103% 89%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NT-ProBNP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 85-92% 79-96% 88-102% 79-102%
EDTA plasma(n=5) 88-97% 96-105% 86-92% 89-97%
Heparin plasma(n=5) 78-92% 86-92% 91-103% 89-97%
전체 세부 정보 보기