DPF2; BAF45D; REQ; UBID4; Zinc finger protein ubi-d4; Apoptosis response zinc finger protein; BRG1-associated factor 45D; BAF45D; D4; zinc and double PHD fingers family 2; Protein requiem
Applications:
WB;IHC;IF;ELISA
Recommended Dilutions:
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Immunogen:
The antiserum was produced against synthesized peptide derived from human REQU. AA range:151-200
Storage:
-20°C/1 year
Storage:
Rabbit
Clonality:
Polyclonal
Isotype:
IgG
Concentration:
1 mg/ml
Observed Band:
44kD
GeneID（Human）:
5977
Human Swiss-Prot No:
Q92785
Cellular localization:
Nucleus . Cytoplasm .
Background:
The protein encoded by this gene is a member of the d4 domain family, characterized by a zinc finger-like structural motif. This protein functions as a transcription factor which is necessary for the apoptotic response following deprivation of survival factors. It likely serves a regulatory role in rapid hematopoietic cell growth and turnover. This gene is considered a candidate gene for multiple endocrine neoplasia type I, an inherited cancer syndrome involving multiple parathyroid, enteropancreatic, and pituitary tumors. [provided by RefSeq, Jul 2008],
Western Blot analysis of various cells using DPF2 Polyclonal Antibody
Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absor
Immunofluorescence analysis of A549 cells, using REQU Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from HT-29 cells, using REQU Antibody. The lane on the right is blocked with the synthesized peptide.